Abstract
| Language | English |
|---|---|
| Journal | Journal of Physical Chemistry B |
| Early online date | 19 Jan 2017 |
| DOIs | |
| State | E-pub ahead of print - 19 Jan 2017 |
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Keywords
- spectroscopy
- DNA
- Hoechst33258
- minor groove binding
- induced fit
- non-covalent interactions
- ligand
- biomolecule
- molecule-DNA binding
Cite this
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2D-IR spectroscopy shows that optimised DNA minor groove binding of Hoechst33258 follows an induced fit model. / Ramakers, Lennart A. I.; Hithell, Gordon; May, John J.; Greetham, Gregory M.; Donaldson, Paul M.; Towrie, Michael; Parker, Anthony W.; Burley, Glenn; Hunt, Neil.
In: Journal of Physical Chemistry B, 19.01.2017.Research output: Contribution to journal › Article
TY - JOUR
T1 - 2D-IR spectroscopy shows that optimised DNA minor groove binding of Hoechst33258 follows an induced fit model
AU - Ramakers,Lennart A. I.
AU - Hithell,Gordon
AU - May,John J.
AU - Greetham,Gregory M.
AU - Donaldson,Paul M.
AU - Towrie,Michael
AU - Parker,Anthony W.
AU - Burley,Glenn
AU - Hunt,Neil
N1 - “This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jpcb.7b00345.
PY - 2017/1/19
Y1 - 2017/1/19
N2 - The induced fit binding model describes a conformational change occurring when a small molecule binds to its biomacromolecular target. The result is enhanced non-covalent interactions between ligand and biomolecule. Induced fit is well-established for small molecule-protein interactions, but its relevance to small molecule-DNA binding is less clear. We investigate the molecular determinants of Hoechst33258 binding to its preferred A-tract sequence relative to a sub-optimal alternating A-T sequence. Results from 2-dimensional infrared spectroscopy, which is sensitive to H-bonding and molecular structure changes, show that Hoechst33258 binding results in loss of minor groove spine of hydration in both sequences, but an additional perturbation of the base propeller twists occurs in the A-tract binding region. This induced fit maximizes favourable ligand-DNA enthalpic contributions in the optimal binding case and demonstrates that controlling the molecular details that induce subtle changes in DNA structure may hold the key to designing next-generation DNA-binding molecules.
AB - The induced fit binding model describes a conformational change occurring when a small molecule binds to its biomacromolecular target. The result is enhanced non-covalent interactions between ligand and biomolecule. Induced fit is well-established for small molecule-protein interactions, but its relevance to small molecule-DNA binding is less clear. We investigate the molecular determinants of Hoechst33258 binding to its preferred A-tract sequence relative to a sub-optimal alternating A-T sequence. Results from 2-dimensional infrared spectroscopy, which is sensitive to H-bonding and molecular structure changes, show that Hoechst33258 binding results in loss of minor groove spine of hydration in both sequences, but an additional perturbation of the base propeller twists occurs in the A-tract binding region. This induced fit maximizes favourable ligand-DNA enthalpic contributions in the optimal binding case and demonstrates that controlling the molecular details that induce subtle changes in DNA structure may hold the key to designing next-generation DNA-binding molecules.
KW - spectroscopy
KW - DNA
KW - Hoechst33258
KW - minor groove binding
KW - induced fit
KW - non-covalent interactions
KW - ligand
KW - biomolecule
KW - molecule-DNA binding
UR - http://pubs.acs.org/journal/jpcbfk
U2 - 10.1021/acs.jpcb.7b00345
DO - 10.1021/acs.jpcb.7b00345
M3 - Article
JO - Journal of Physical Chemistry B
T2 - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
SN - 1520-6106
ER -