170 Mitochondrial-dependent signalling in vascular smooth muscle cell proliferation

Zuhair Al-Sulti, David Kingsmore, Paul Coats

Research output: Contribution to journalArticle

Abstract

UNLABELLED: A hallmark of vascular disease is the cellular adaptive response characterised by proliferation and migration. Although many studies have identified the signalling pathways involved in cell proliferation and migration (p38, p44/42 MAP Kinase and JNK), the mechanisms initiating cell de-differentiation, proliferation/ apoptosis and migration are yet to be fully elucidated. Mitochondria are one of the organelles that have received growing attention in vascular and pulmonary vascular proliferative disease.(1) Mitochondria are classically known to be responsible for cellular energy production. However growing evidence suggests a role in cell de-differentiation and the fine balance between cell apoptosis and proliferation.(2) The aim of this work was to expand our understanding of the potential role of mitochondrial and possible influences on vascular proliferative signalling mechanism. Our initial results confirmed that inhibiting mitochondrial fission process using DRP1 inhibitor MDivi-1 resulted in a significant inhibition of vascular smooth muscle cells proliferation stimulated by both insulin (p < 0.05) and foetal calf serum (p < 0.05).(3) Western blot results showed that the phosphorylation of p44/42 was unaffected by MDivi-1 treatment, however, phosphorylation of Akt, cyclin D and 4EBP1 were reduced when the cells were treated with 10μM of MDivi-1. Cell cycle analysis was also conducted and the results showed a concentration dependent cell cycle arrest at G2/M phase (p < 0.05). These results further support the concept that mitochondria play an important role in vascular smooth muscle cell proliferation independent of the classic pro-mitogenic p44/42 MAPK signalling pathway and highlight a potential role for PI3k-Akt-mTOR-dependent signalling. However, the exact point where mitochondria feature in the mechanistic signalling pathway has yet to be fully elucidated.

REFERENCES: Dromparis P, et al. The role of mitochondria in pulmonary vascular remodeling. Journal of molecular medicine (Berlin, Germany) 2010;88:1003-1010 Galluzzi L, et al. Mitochondrial control of cellular life, stress, and death. Circ. Res. 2012;111:1198-1207 Chalmers S, et al. Mitochondrial motility and vascular smooth muscle proliferation. Arterioscler Thromb Vasc Biol 2012;32:3000-3011.

LanguageEnglish
Article numberA97
JournalHeart
Volume100
Issue numberSuppl. 3
DOIs
Publication statusPublished - Jun 2014

Fingerprint

Vascular Smooth Muscle
Smooth Muscle Myocytes
Mitochondria
Cell Proliferation
Vascular Diseases
Blood Vessels
Cell Differentiation
Phosphorylation
MAP Kinase Kinase 4
Mitochondrial Dynamics
Apoptosis
Cyclin D
Molecular Medicine
Lung
Mitogen-Activated Protein Kinase 3
G2 Phase
Berlin
Cell Cycle Checkpoints
Psychological Stress
Organelles

Keywords

  • vascular disease
  • smooth muscle cell proliferation
  • mitochondria

Cite this

Al-Sulti, Zuhair ; Kingsmore, David ; Coats, Paul. / 170 Mitochondrial-dependent signalling in vascular smooth muscle cell proliferation. In: Heart . 2014 ; Vol. 100, No. Suppl. 3.
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170 Mitochondrial-dependent signalling in vascular smooth muscle cell proliferation. / Al-Sulti, Zuhair; Kingsmore, David; Coats, Paul.

In: Heart , Vol. 100, No. Suppl. 3, A97, 06.2014.

Research output: Contribution to journalArticle

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N2 - UNLABELLED: A hallmark of vascular disease is the cellular adaptive response characterised by proliferation and migration. Although many studies have identified the signalling pathways involved in cell proliferation and migration (p38, p44/42 MAP Kinase and JNK), the mechanisms initiating cell de-differentiation, proliferation/ apoptosis and migration are yet to be fully elucidated. Mitochondria are one of the organelles that have received growing attention in vascular and pulmonary vascular proliferative disease.(1) Mitochondria are classically known to be responsible for cellular energy production. However growing evidence suggests a role in cell de-differentiation and the fine balance between cell apoptosis and proliferation.(2) The aim of this work was to expand our understanding of the potential role of mitochondrial and possible influences on vascular proliferative signalling mechanism. Our initial results confirmed that inhibiting mitochondrial fission process using DRP1 inhibitor MDivi-1 resulted in a significant inhibition of vascular smooth muscle cells proliferation stimulated by both insulin (p < 0.05) and foetal calf serum (p < 0.05).(3) Western blot results showed that the phosphorylation of p44/42 was unaffected by MDivi-1 treatment, however, phosphorylation of Akt, cyclin D and 4EBP1 were reduced when the cells were treated with 10μM of MDivi-1. Cell cycle analysis was also conducted and the results showed a concentration dependent cell cycle arrest at G2/M phase (p < 0.05). These results further support the concept that mitochondria play an important role in vascular smooth muscle cell proliferation independent of the classic pro-mitogenic p44/42 MAPK signalling pathway and highlight a potential role for PI3k-Akt-mTOR-dependent signalling. However, the exact point where mitochondria feature in the mechanistic signalling pathway has yet to be fully elucidated.REFERENCES: Dromparis P, et al. The role of mitochondria in pulmonary vascular remodeling. Journal of molecular medicine (Berlin, Germany) 2010;88:1003-1010 Galluzzi L, et al. Mitochondrial control of cellular life, stress, and death. Circ. Res. 2012;111:1198-1207 Chalmers S, et al. Mitochondrial motility and vascular smooth muscle proliferation. Arterioscler Thromb Vasc Biol 2012;32:3000-3011.

AB - UNLABELLED: A hallmark of vascular disease is the cellular adaptive response characterised by proliferation and migration. Although many studies have identified the signalling pathways involved in cell proliferation and migration (p38, p44/42 MAP Kinase and JNK), the mechanisms initiating cell de-differentiation, proliferation/ apoptosis and migration are yet to be fully elucidated. Mitochondria are one of the organelles that have received growing attention in vascular and pulmonary vascular proliferative disease.(1) Mitochondria are classically known to be responsible for cellular energy production. However growing evidence suggests a role in cell de-differentiation and the fine balance between cell apoptosis and proliferation.(2) The aim of this work was to expand our understanding of the potential role of mitochondrial and possible influences on vascular proliferative signalling mechanism. Our initial results confirmed that inhibiting mitochondrial fission process using DRP1 inhibitor MDivi-1 resulted in a significant inhibition of vascular smooth muscle cells proliferation stimulated by both insulin (p < 0.05) and foetal calf serum (p < 0.05).(3) Western blot results showed that the phosphorylation of p44/42 was unaffected by MDivi-1 treatment, however, phosphorylation of Akt, cyclin D and 4EBP1 were reduced when the cells were treated with 10μM of MDivi-1. Cell cycle analysis was also conducted and the results showed a concentration dependent cell cycle arrest at G2/M phase (p < 0.05). These results further support the concept that mitochondria play an important role in vascular smooth muscle cell proliferation independent of the classic pro-mitogenic p44/42 MAPK signalling pathway and highlight a potential role for PI3k-Akt-mTOR-dependent signalling. However, the exact point where mitochondria feature in the mechanistic signalling pathway has yet to be fully elucidated.REFERENCES: Dromparis P, et al. The role of mitochondria in pulmonary vascular remodeling. Journal of molecular medicine (Berlin, Germany) 2010;88:1003-1010 Galluzzi L, et al. Mitochondrial control of cellular life, stress, and death. Circ. Res. 2012;111:1198-1207 Chalmers S, et al. Mitochondrial motility and vascular smooth muscle proliferation. Arterioscler Thromb Vasc Biol 2012;32:3000-3011.

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