α2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an α2β1 integrin dependent mechanism: an in vitro and in vivo study

Neill J. Turner, Michael O. Murphy, Cay M. Kielty, Adrian Shuttleworth, Richard A. Black, Martin J. Humphries, Michael G. Walker, Ann E. Canfield

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Essential to tissue-engineered vascular grafts is the formation of a functional endothelial monolayer capable of resisting the forces of blood flow. This study targeted {alpha}2(VIII) collagen, a major component of the subendothelial matrix, and examined the ability of and mechanisms by which endothelial cells attach to this collagen under static and dynamic conditions both in vitro and in vivo. These studies demonstrate that {alpha}2(VIII) collagen has the potential to improve both initial cell attachment and retention of endothelial cells on vascular grafts in vivo, which opens new avenues of research into the development of single-stage endothelialized prostheses and the next generation of tissue-engineered vascular grafts.
    LanguageEnglish
    Pages820-829
    Number of pages9
    JournalCirculation
    Volume114
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Integrins
    Blood Vessel Prosthesis
    Collagen
    Endothelial Cells
    Prostheses and Implants
    Transplants
    Research
    In Vitro Techniques

    Keywords

    • circulation
    • bioengineering
    • biomaterials
    • cells
    • vacular grafts
    • heart

    Cite this

    Turner, Neill J. ; Murphy, Michael O. ; Kielty, Cay M. ; Shuttleworth, Adrian ; Black, Richard A. ; Humphries, Martin J. ; Walker, Michael G. ; Canfield, Ann E. / α2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an α2β1 integrin dependent mechanism: an in vitro and in vivo study. In: Circulation. 2006 ; Vol. 114. pp. 820-829.
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    abstract = "Essential to tissue-engineered vascular grafts is the formation of a functional endothelial monolayer capable of resisting the forces of blood flow. This study targeted {alpha}2(VIII) collagen, a major component of the subendothelial matrix, and examined the ability of and mechanisms by which endothelial cells attach to this collagen under static and dynamic conditions both in vitro and in vivo. These studies demonstrate that {alpha}2(VIII) collagen has the potential to improve both initial cell attachment and retention of endothelial cells on vascular grafts in vivo, which opens new avenues of research into the development of single-stage endothelialized prostheses and the next generation of tissue-engineered vascular grafts.",
    keywords = "circulation, bioengineering, biomaterials, cells, vacular grafts, heart",
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    α2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an α2β1 integrin dependent mechanism: an in vitro and in vivo study. / Turner, Neill J.; Murphy, Michael O.; Kielty, Cay M.; Shuttleworth, Adrian; Black, Richard A.; Humphries, Martin J.; Walker, Michael G.; Canfield, Ann E.

    In: Circulation, Vol. 114, 2006, p. 820-829.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - α2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an α2β1 integrin dependent mechanism: an in vitro and in vivo study

    AU - Turner, Neill J.

    AU - Murphy, Michael O.

    AU - Kielty, Cay M.

    AU - Shuttleworth, Adrian

    AU - Black, Richard A.

    AU - Humphries, Martin J.

    AU - Walker, Michael G.

    AU - Canfield, Ann E.

    PY - 2006

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    AB - Essential to tissue-engineered vascular grafts is the formation of a functional endothelial monolayer capable of resisting the forces of blood flow. This study targeted {alpha}2(VIII) collagen, a major component of the subendothelial matrix, and examined the ability of and mechanisms by which endothelial cells attach to this collagen under static and dynamic conditions both in vitro and in vivo. These studies demonstrate that {alpha}2(VIII) collagen has the potential to improve both initial cell attachment and retention of endothelial cells on vascular grafts in vivo, which opens new avenues of research into the development of single-stage endothelialized prostheses and the next generation of tissue-engineered vascular grafts.

    KW - circulation

    KW - bioengineering

    KW - biomaterials

    KW - cells

    KW - vacular grafts

    KW - heart

    UR - http://dx.doi.org/10.1161/CIRCULATIONAHA.106.635292

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