Macromolecular construction of DNA networks directed by the fluorous effect

This project is focused on:
(i) Development of robust and orthogonal molecular recognition modalities at DNA nanostructure interfaces. Auxiliary molecular recognition modes which complement W-C base-pairing would be highly desirable as they could direct novel assembly processes not readily available using W-C base-pairing in isolation;
(ii) Systematic characterisation of the kinetics and thermodynamics of DNA nanostructure interfaces. A detailed understanding of the kinetic and thermodynamic parameters influencing hierarchical assembly will be essential to optimize desired assembly pathways whilst minimizing incorrectly formed complexes;
(iii) Development of methodology to integrate micron-scale, 3D DNA nanostructure networks in defined surface patterns

This work is focused on combining the fluorous effect with DNA origami to develop new ways of controlling DNA assembly.