EPSRC Doctoral Training Grant - DTA, University of Strathclyde | Russell, Ben Allan

  • Chen, Yu (Principal Investigator)
  • Mulheran, Paul (Co-investigator)
  • Russell, Ben Allan, (Research Co-investigator)

Project: Research Studentship - Internally AllocatedResearch Studentship (Internally Allocated)

Key findings

From Fluorescence and computational studies of synthesised Human Serum Albumin Gold Nanoclusters the location of nanocluster nucleation and growth in the protein was found to be centred in the second domain, located near one of the major drug binding sites of the protein.

From hydrodynamic and adsorption technique studies it was found that both Lysozyme and Human Serum Albumin protein encapsulated Gold Nanoclusters form larger aggregates than the proteins natural form, as well as altering a number of important characteristics such as the isoelectric point and the viscosity.

From further fluorescence studies it was found that the gold nanocluster in Human Serum Albumin blocks the ability of the protein to bind to drug molecules at Sudlow site I of the protein. This was found during a series of experiments using the fluorescent drug Warfarin.
StatusFinished
Effective start/end date1/10/132/06/17

Funding

  • EPSRC (Engineering and Physical Sciences Research Council): £14,009.00

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Differential thermal analysis
Nanoclusters
Serum Albumin
Gold
Proteins
Fluorescence
Pharmaceutical Preparations
Warfarin
Muramidase
Nucleation
Hydrodynamics
Binding Sites
Viscosity
Adsorption
Molecules
Experiments