The inactivation of highly resilient Clostridium difficile spores using 405 nm light and the investigation into mechanisms to enhance spore susceptability.
The inactivation of highly resilient Clostridium difficile spores using violet-blue light at a wavelength of 405 nm light.
This study to date has demonstrated the efficacy of 405 nm violet-blue light for the inactivation of C. difficile vegetative cells and spores, and has compared the inactivation kinetics of the choice strain for this study to that of other C. difficile ribotypes. This study has further demonstrated the potential synergistic oxidative effect observed upon combination of 405 nm light with chlorine based disinfectants recommended for sporicidal cleaning. These high strength disinfectants have various drawbacks associated with the concentrations required for sporicidal kill, including their corrosive nature and the release of irritating vapour - this study has provided a potential solution for enhanced sporicidal kill at lowered chlorine concentrations. The enhanced sensitivity of C. difficile spores to 405 nm light upon the initiation of germination has been demonstrated.
|Effective start/end date||1/10/13 → 28/09/17|
- EPSRC (Engineering and Physical Sciences Research Council): £9,957.00