Enhancing industrial production of succinate in Corynebacterium glutamicum

C. glutamicum is already used for the commercial production of amino acids. It is robust, grows quickly, genome sequences have been published and molecular genetic tools are available. It is the ideal ‘organism chassis’ on which to develop ‘green succinate’ production. Three C4-diC transporters have been reported and there are also four SLC26 genes in the genome – so far not investigated. The The proposition is to characterise all to determine which exporter is most appropriate to manipulate to enhance production of green succinate. Then to construct a second generation strain that has its metabolism altered to predispose it to succinate over-production, taking advantage of the availability of the exporter to excrete it from the cell.

£10,000 allocated