Project Details
Description
Project outline
Hypertension is a major risk factor for the development of cardiovascular diseases. Many individuals with essential hypertension are salt‐sensitive [1,2]. The exact mechanisms underlying salt-sensitive hypertension remain elusive. Preliminary data have demonstrated that in in vivo, a high salt diet (HSD) leads to vascular hypercontractility and increased blood pressure. In vitro, high sodium medium behaves as a pro-oxidant agent in several cells, which may be associated with cardiac and renal fibrosis. In hypertension, changes in the composition of cardiac tissue leads to structural remodelling of the myocardium, which may contribute to cardiac complications [3]. Hypertension also results in renal interstitial fibrosis, which can cause renal damage, where high salt diet plays a role [4].
Project aim
In this project we aim to investigate whether heart and kidney isolated from C57BL/6 mice treated for 5 weeks with high salt diet (4%) develop fibrosis.
Methods
Histological visualization of kidneys, hearts and aorta will be assessed by haematoxylin and eosin (H&E) staining. Fibrosis will be assessed by picrosirius red staining, a histological technique used to visualize the distribution of collagen in tissue sections and a well establish fibrosis marker.
Hypertension is a major risk factor for the development of cardiovascular diseases. Many individuals with essential hypertension are salt‐sensitive [1,2]. The exact mechanisms underlying salt-sensitive hypertension remain elusive. Preliminary data have demonstrated that in in vivo, a high salt diet (HSD) leads to vascular hypercontractility and increased blood pressure. In vitro, high sodium medium behaves as a pro-oxidant agent in several cells, which may be associated with cardiac and renal fibrosis. In hypertension, changes in the composition of cardiac tissue leads to structural remodelling of the myocardium, which may contribute to cardiac complications [3]. Hypertension also results in renal interstitial fibrosis, which can cause renal damage, where high salt diet plays a role [4].
Project aim
In this project we aim to investigate whether heart and kidney isolated from C57BL/6 mice treated for 5 weeks with high salt diet (4%) develop fibrosis.
Methods
Histological visualization of kidneys, hearts and aorta will be assessed by haematoxylin and eosin (H&E) staining. Fibrosis will be assessed by picrosirius red staining, a histological technique used to visualize the distribution of collagen in tissue sections and a well establish fibrosis marker.
| Status | Not started |
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