Bioinspired, Spatially-Organized Nanostructures for Enzyme Encapsulation and Biocatalysis

Project: Research

Description

This project aims to develop bioinspired, synthetic nanoporous structures that can control biomacromolecular transport through nanopores, and hence enable a novel strategy for enzyme encapsulation and efficient biocatalysis.

It will enable a practical format for enzyme catalysis, and make efficient and environmentally friendly catalysis routine. Enzyme immobilization has long been proposed to circumvent the stability, recovery and reuse problems of using enzymes. However covalent surface attachment generally lowers activity, due to binding site occlusion and protein denaturation. Conventional enzyme encapsulation also suffers from either low enzyme loading or high rates of escape due to the size mismatch between enzymes and the pore size of the host support. This project will meet the need for a new approach to enzyme immobilization that maintains enzyme activity and enables convenient reuse and recovery.

Other than enabling enzyme encapsulation, this research will also provide fundamental knowledge to the general problem of molecular transport through nanopores and enable fouling resistant, high flux ultrafiltration and other applications that require molecular separation.
StatusActive
Effective start/end date13/01/14 → …