Projects per year
PROTEIN S-ACYLATION IN HEALTH AND DISEASE
S-acylation (aka palmitoylation), the reversible attachment of fatty acids onto cysteine residues, regulates a diverse array of proteins and impacts fundamental cellular processes such as signalling, membrane traffic, communication, and growth and division. Defects in S-acylation are linked with cancer, diabetes, and CNS disorders such as intellectual disability, epilepsy, Huntington’s disease and neuronal ceroid lipofuscinosis. In addition, S-acylation is required for infection and virulence of some viruses and parasites. As a result, there is growing interest in the therapeutic potential of targeting the S-acylation machinery, with a major goal being the development of isoform-selective inhibitors against the 23 zDHHC S-acyltransferase enzymes.
RESEARCH IN THE CHAMBERLAIN LAB
The research that we are undertaking aims to unravel the multitude of functions that S-acylation plays in cellular pathways, in particular, signalling and membrane traffic. In addition, we aim to understand how defects in S-acylation contribute to disorders such as intellectual disability, epilepsy, neurodegeneration, cancer and diabetes. Through this work we hope to identify novel drug targets and new drug treatments for these conditions. We use a wide range of techniques including chemical biology (click chemistry), confocal microscopy, proteomics, and behavioural analyses.
Our research falls into two major programmes:
1. S-acylation and cell function in health and disease
(i) How does dynamic S-acylation of key signalling and trafficking proteins regulate cell pathways and how does disruption of this process cause disease?
(ii) What are the molecular effects of S-acylation that underlie protein regulation?
(iii) Does acyl chain heterogeneity provide functionally distinct pools of the same protein?
2. The zDHHC family of S-acyltransferases
(i) What are the substrate networks of individual zDHHC enzymes, and how is enzyme-substrate specificity encoded?
(ii) How do zDHHC enzymes select specific acyl-CoAs from a mixed population?
(iii) How do multiple zDHHC enzyme isoforms coordinate protein S-acylation at the level of a single intracellular organelle?
(iv) What are the cellular and molecular changes that underlie disease phenotypes caused by ZDHHC mutations?
(v) Can we develop isoform-selective chemical modulators of the zDHHC enzyme family?
We would be delighted to hear from post-doctoral researchers interested in developing fellowhsip proposals that can be hosted in our lab. We can offer support and mentorship for fellowship applications.
We are also happy to speak with prospective PhD students who have secured funding.
All enquiries can be directed to firstname.lastname@example.org
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
Doctor of Philosophy, University of Liverpool
Award Date: 1 Jan 1998
Bachelor of Science, University of Edinburgh
Award Date: 1 Jan 1994
- lipid raft
- membrane traffic
- 1 Similar Profiles
Dive into details
Select a country/territory to view shared publications and projects
S-Acylation of transmembrane proteins in the early secretory pathway
BBSRC (Biotech & Biological Sciences Research Council)
1/02/23 → 31/01/26
Targeting The ZDHHC9-Gcp16 Interaction As A Novel Therapeutic Strategy In Cancer And Neurological Disorders
Chamberlain, L. & Tomkinson, N.
Genentech, Medical Research Scotland
1/10/21 → 30/09/25
Project: Research - Studentship
S-acylation of Sprouty and SPRED proteins by the S-acyltransferase zDHHC17 involves a novel mode of enzyme-substrate interactionButler, L., Locatelli, C., Allagioti, D., Lousa, I., Lemonidis, K., Tomkinson, N. C. O., Salaun, C. & Chamberlain, L. H., 31 Jan 2023, In: Journal of Biological Chemistry. 299, 1, 13 p., 102754.
Research output: Contribution to journal › Article › peer-reviewOpen AccessFile9 Downloads (Pure)
Development of a novel high-throughput screen for the identification of new inhibitors of protein S-acylationSalaun, C., Takizawa, H., Galindo, A., Munro, K. R., McLellan, J., Sugimoto, I., Okino, T., Tomkinson, N. C. O. & Chamberlain, L. H., 31 Oct 2022, In: Journal of Biological Chemistry. 298, 10, 22 p., 102469.
Research output: Contribution to journal › Article › peer-reviewOpen AccessFile
Christine Salaun (Host) & Luke Chamberlain (Host)21 Sep 2022
Activity: Hosting a visitor types › Hosting an academic visitor
UKRI Medical Research Council (External organisation)
Luke Chamberlain (Advisor)1 Sep 2021 → 31 Jan 2022
Activity: Membership types › Membership of committee
Leica SP8 Confocal MicroscopeStrathclyde Institute Of Pharmacy And Biomedical Sciences