This dataset is a progression from the recent work on understanding localisation and uptake of gold nanoparticles inside multicellular tumour spheroids (MTS) as a bridge between 2D cellular monolayers and in vivo tumours. We have now employed an in vivo chick embryo model and used SERS to locate the distribution of the nanoparticles throughout the tumour both intact and sectioned and then detected the nanoparticles in the tumour through a barrier using SESORS