Data for "Unmixing hyperspectral SRS images in the cell-silent region of the Raman spectrum using phasor analysis"

Dataset

Description

This dataset was generated for the publication of a research article of the same title and investigates the use of spectral phasor analysis for multicomponent imaging in the cell-silent region of the Raman spectrum using stimulated Raman scattering (SRS) microscopy. SRS images were acquired using a Leica multiphoton microscope. SRS images are provided in .tif file format with post-processing performed using ImageJ. Spectral phasor analysis was performed using a plug-in for ImageJ (FiJi). Manual segmentation of the phasor plot was used to identify regions of interest within the hyperspectral SRS image dataset.

Embargo end date 24/04/26
Date made available12 May 2025
PublisherUniversity of Strathclyde
Date of data production1 Sept 2024 - 24 Apr 2025

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