Single atom imaging requires discrimination of weak photon count events above background and has typically been performed using either EMCCD cameras, photomultiplier tubes or single photon counting modules. sCMOS provides a cost effective and highly scalable alternative to other single atom imaging technologies, offering fast readout and larger sensor dimensions. We demonstrate the first single atom resolved imaging of two site-addressable single atom traps separated by 10 μm using an sCMOS camera, offering a competitive signal-to-noise ratio at intermediate count rates to allow high fidelity readout discrimination (error < 10−6) and sub-μm spatial resolution for applications in quantum technologies.