Data for "Reactivity profiling for high yielding Ynamine-tagged oligonucleotide click chemistry bioconjugations"

This is the readme file for the publication titled: "Reactivity profiling for high yielding Ynamine-tagged oligonucleotide click chemistry bioconjugations"


Folder structure:
* The data is split into two main folders: main text data and SI
* Main text data is split into Figures
* Figures contain HPLC data presented in the figure as pdf reports of the HPLC chromatograms; a readme file is added to each folder specifying which reaction codes correspond to which reactions
* main text data also contains an OriginLab file which contains all the processed figures of the main text
* SI data is organised into two folders "Compounds" and "Additional Figures"
* The "Compounds" folder contains characterisation data for the synthesised oligos and protein conjugates labelled by compound number as presented in the paper
* Characterisation consists of a purity HPLC trace and MALDI spectrum for oligonucleotides, an ESI HRMS data for protein conjugates and 1H/13C-NMr for compound S1
* The "Additional Figures" folder contains the data for the additional figures in the SI as well as the OriginLab file which contains all the processed data for the figures in the SI
* The folder is split into SI Figures
* Each folder contains HPLC data presented in the figure as pdf reports of the HPLC chromatograms; a readme file is added to each folder specifying which reaction codes correspond to which reactions


Software needed to view files:
* a pdf viewer is needed to view HPLC chromatogram data
* A NMR software (such as Mestrenova) is needed to view the raw NMR data files; processed Mestrenova files are also provided alongside a pdf of the processed data
* Figures were constructed using OriginLab Pro 2024; origin files are also provided in the main text data and SI; alternatively plots can be reconstructed by extracting the peak area from the provided HPLC chromatograms
* HRMS data can be viewed and processed by the open source software UniDec freely available at: https://github.com/michaelmarty/UniDec