Data for: "Label-free imaging of thick tissue at 1550  nm using a femtosecond optical parametric generator"

  • Johanna Trägardh (Contributor)
  • Gillian Robb (Creator)
  • Kamal K. E. Gadalla (Creator)
  • Stuart Cobb (Creator)
  • Christopher Travis (Creator)
  • Gian-Luca Oppo (Contributor)
  • Gillian McConnell (Creator)

Dataset

Description

We have developed a simple wavelength-tunable optical parametric generator (OPG), emitting broadband ultrashort pulses with peak wavelengths at 1530–1790 nm, for nonlinear label-free microscopy. The OPG consists of a periodically poled lithium niobate crystal, pumped at 1064 nm by a ultrafast Yb:fiber laser with high pulse energy. We demonstrate that this OPG can be used for label-free imaging, by third-harmonic generation, of nuclei of brain cells and blood vessels in a >150  μm thick brain tissue section, with very little decay of intensity with imaging depth and no visible damage to the tissue at an incident average power of 15 mW.

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Date made available16 Dec 2022
Publisherfigshare

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