Data associated to the PhD thesis "Next-generation transcriptional activators for the treatment of Friedreich's Ataxia"

The data herein are related to the PhD thesis "Next-generation transcriptional activators for the treatment of Friedreich's Ataxia". The overall aim of this project was the development of synthetic activators with improved potency and selectivity. This was achieved by focusing on the optimization of the DNA-binding domain of the synthetic activator which is represented by a pyrrole-imidazole polyamide. A synthetic campaign was applied to identify an improved DNA binder. The data herein describes the synthesis of a suite of polyamides as well as the evaluation of their binding properties through a combination of biophysical and structural methods. All the data are saved within folders and are organized in Chapters that correspond to thesis Chapters. Compounds are named according to thesis nomenclature. Correspondance between thesis codes and ELN codes is provided. Each folder contains a range of data as Word, Excel, PowerPoint, text files, Origin and specific program files. All NMR data were acquired on Bruker spectrometers and processed with MestReNova. HPLC data were acquired on a Dionex Ultimate 3000 and processed with Chromeleon sofware. UV melt analysis was carried out on a Shimadzu UV spectrometer UV 1800 and processed with OriginLab software. The biophysical assay was performed on a heliX® instrument (Dynamic Biosensors) and data processed with the heliX sofware.