Description
Nanodiamond is a fluorescent nanoparticle that doesn’t photobleach over time or with high excitation, it is also a brilliant tool for biological imagining due to its low cytotoxicity and its sub-diffraction size allowing high spatio-temporal resolution enabling biotracking and biosensing. Nanodiamond has been used as a marker for various super resolution microscopy techniques, such as STORM, STED and SLIM, moreover, nanodiamond is used in sensing schemes for thermometry and magnetometry.As a complementary method we have constructed a simple widefield system using both total internal reflection fluorescence and Epifluorescent microscopy techniques to facilitate sample testing, TIRF excitation of nanodiamond in light sensitive materials and sensing schemes that could be implemented in a super resolution system.
This talk would aim to give an overview of the complementary aspects of diffraction limited systems with super resolution systems for the preparation and measurement of nanodiamond in biological systems, further the steps we have taken to refine measurements in the diffraction limited system such as Bayesian analysis and computational super resolution radial fluctuations.
| Period | 7 Jun 2023 |
|---|---|
| Event title | Super-resolution Workshop2023 |
| Event type | Workshop |
| Location | Leeds, United KingdomShow on map |
| Degree of Recognition | International |