A Multimodal Microscope for Improved Contrast Imaging and Optically Detected Magnetic Resonance of NV- Centres in Nanodiamond

Activity: Talk or presentation typesOral presentation

Description

Nanodiamonds containing nitrogen vacancy (NV-) centres are fluorescent nanoparticles that do not photobleach over time, even when illuminated with high optical powers. They are small in size, and have very low cytotoxicity. These properties make nanodiamonds with NV- centres ideal for biological imaging, allowing high spatio-temporal resolution biotracking and biosensing. Nanodiamond with NV- centres offer a further advantage for biological imaging through their ability to provide a readout of relative changes in magnetic field and temperature using optically detected magnetic resonance (ODMR). They have been used as a marker in various super resolution microscopy techniques but these methods are highly complex and are not routinely available in biology labs.
To lower the barrier to entry to this useful technology, we have developed a multimodal microscope specifically for imaging of nanodiamonds in biological specimens using low-cost components where possible. Our new imaging platform incorporates both widefield epifluorescence illumination for more accessible characterization of nanodiamonds and measurement of ODMR prior to their application in biological systems, and total internal reflection fluorescence for improved contrast ODMR and bioimaging using fluorescent nanodiamonds as reporters.
I will give an overview of the system design, showing imaging data obtained using the platform, and I will present progress on measurements in biological systems.
Period7 Nov 2023
Event titleScottish Microscopy Society Annual Symposium
Event typeConference
LocationDundee, United KingdomShow on map
Degree of RecognitionNational